RESEARCH EXPERIENCE

Characterizing the function of Bovine herpesvirus 1 UL51 protein in viral capsid envelopment, de-envelopment and secondary envelopment.

Research Team Leader: Jan 2013- Feb 2016

Construction and characterization of Bovine herpesvirus 1(BoHV-1) bacterial artificial Chromosome (BAC).
Construction and characterization of BoHV-1 UL51 mutant using BAC recombineering and transgenesis techniques.
Construction and characterization of hemagglutinin (HA) tagged viruses UL51 mutant using BAC recombineering and transgenesis techniques.
Growth kinetics, Transmission Electron Microscopy (TEM) and immunefluorescence analysis of UL51 mutant and HA tagged viruses.
Cellular localization and Immunoprecipitation of pUL51 bounded proteins.
In vivo characterization of UL51 mutant virus.

Construction, virulence and protective efficacy of Bovine herpesvirus 1 △gG/△tk/△gE and △gG/△tk/△gN gene-deleted vaccine.

Research Team Member, Oct 2012 - Jan 2014

Construction of Bovine herpesvirus 1 △gG/△tk/△gE and △gG/△tk/△gN gene-deleted mutants
Compared their virulence and protective efficacy with Bovine herpesvirus 1 △gG/△tk mutant in calves and rabbits.

The optimization of immunization approaches and development of Bovine herpesvirus 1 gG-/tk- marker vaccine.

Research Team Member, Oct 2012 - Jan 2014

Comparison of immune response and protection using different vaccination routes of BoHV-1 △gG/△tk vaccine.
gB and gG protein expression and development of gB-ELISA and gG-ELISA for discriminating infected from vaccinated animals

Prevalence study and genetic typing of bovine viral diarrhea virus (BVDV) in four bovine species in China.

Research Team Member, Oct 2012 - Jun 2014

Collected serum samples from dairy cattle, beef cattle, yaks, and water buffalo from eight provinces of China during 2010 to 2013.
Samples detected by using commercial BVDV antibody (Ab) and antigen (Ag) detection kits and RT-PCR based on the 5’-UTR and Npro gene sequencing.
Analysis of the data and genetic typing of bovine viral diarrhea virus (BVDV)

Construction and immunogenicity of a recombinant Bovine herpesvirus 1 expressing glycoprotein E2 of bovine viral diarrhoea virus and glycoprotein D of Bovine herpesvirus 1.

Research Team Member, June 2013 – June 2016

Cloning of wild-type Bovine herpesvirus 1 and BoHV-1 △gG/△tk genome as an infectious BAC.
Pre-expressed gD-E2 fusion gene in 293T cell drivered by difference promoter.
Inserted gD-E2 fusion gene into difference site of BoHV-1 BAC
Reconstituted recombinant virus and analyzed native antigenic properties of gD-E2
Study the immunogenicity and protective efficacy of recombinant virus in calves.