Andrea Cacciamali has a degree in Biomedical Laboratory Techniques since 2005, from the University of Brescia, and in Biological Sciences since 2013, from the University of Milan, with a specialisation in Applied Biology for Biomedical Research. Since 2022, he has been a specialist in Clinical Pathology and Clinical Biochemistry at the University of Brescia. From January 2003 to October 2005, he was a university intern at the Paediatric Bone Marrow Transplantation Laboratory of the Spedali Civili of Brescia where he performed post-graduate activities until September 2006 with a professional service contract. From August 2014 to July 2015 he worked at the National Institute of Tumours in Milan as a research fellow. Since 2015, he has been working at the Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna first as a research fellow, within the scope of several research projects, and since December 2019 he has been employed on a fixed-term basis, at the Department of Production and Control of Biological Materials, with the title of Health Researcher in the "Research Pyramid" programme. Since 1 April 2023, he has been a fixed-term biologist manager in the Vaccine and Reagent Production Laboratory. The skills developed over the years in the laboratory concern the use of 2D and 3D cell culture models, transfection and gene silencing techniques using RNA-interferencing, assays for proliferation, cytotoxicity, cell invasion and migration, immunofluorescence, nucleic acid extraction from biological and environmental matrices, Real-Time PCR and Western blot for studying gene expression. The research activity carried out at IZSLER has mainly focused on the development of alternative methods, with reference to the 3R principle, the development of direct and indirect diagnostic methods for monitoring the health of laboratory animals and the writing of research projects and scientific articles on the application of alternative methods to animal experimentation.