Prakash Biswas

Mr
Prakash
Biswas

PhD Scholar
Indian Institute of Science Education and Research Kolkata
Biography
I am Prakash Biswas, a senior PhD scholar under the supervision of Dr. Amirul Islam Mallick, Department of Biological Sciences, IISER Kolkata. My research work is based on understanding the role of Campylobacter jejuni's adhesion proteins in differential host responses and beyond and developing a live vector-based multivalent vaccine against cecal colonization of Campylobacter jejuni in commercial chickens. I have completed my bachelor's degree in microbiology (62.38%, 2018) from Barrackpore Rastraguru Surendranath College, WestBengal State University, and my master's degree in microbiology (74.9%, 2020) from Bidhannagar College, West Bengal State University. Currently, I am pursuing my PhD (2021-present) in IISER Kolkata. I have experience as being teaching assistant in courses like Immunology and Cell Biology and Imaging Laboratory from IISER Kolkata. Best poster award at the 11th INTERNATIONAL CONFERENCE LABORATORY ANIMAL SCIENTISTS’ ASSOCIATION (LASA) INDIA, LASACON 2023 held in Bengaluru, Organized by Laboratory Animal Scientists' Association (LASA), India on "Advancements of 3R's in Biomedical Research, Laboratory Animal Science and Welfare: International Perspectives". Qualified “State Eligibility Test (SET) for Assistant Professor, West Bengal” in 2020. Qualified “Graduate Aptitude Test in Engineering (GATE)” in 2020. Qualified as “Junior Research Fellow (JRF) in Joint CSIR-UGC NET examination” Dec 2019. Scientific skills: Molecular biology: Molecular cloning, Expression, purification of Proteins, Sequence analysis, Western blot, Semi-quantitative RT-PCR. Cell Culture: Experience in mammalian cell lines. Cytotoxicity assay. Immunology: Fluorescence microscopy, Flow cytometric analysis, ELISA, Ligand Receptor Interaction assay, Spleenocytes Proliferation assay, NO production assay, In vitro binding assay, In vitro Neutralization assay. Microbiology: Protection assay, Adherence, and invasion assay. Instrumentation: Circular dichroism, Isothermal Titration Calorimetry, Surface Plasmon Resonance, Confocal microscopy, PCR, Centrifuge, Gel Filtration assay. Animal Handling: Hands-on experience in Mice and chicken handling and sample processing.
Research interests
My current research work is based on understanding the role of bacterial adhesion proteins in differential host responses and beyond. Campylobacter jejuni (C. jejuni) is one of the major causes of diarrhoeal diseases in humans. Although C. jejuni has a broad host range, chickens remain the primary source of human infection. Intriguingly, C. jejuni naturally inhabits chicken’s gut with little or no sign of disease or illness. At the same time, in the case of humans, C. jejuni causes acute gastroenteritis and several autoimmune disorders. Among the major factors particularly involved in bacterial adherence and colonization, SECPs (CadF, Jlpa, FlpA) of C. jejuni represent a key element for adhesion and invasion. However, the direct role of SECPs in mediating differential responses in humans and chickens is incomprehensible. Based on this problem, my ongoing PhD research is based on two major objectives: 1) To investigate the role of key adhesion proteins of C. jejuni in mediating differential host responses. 2) To develop a live vector-based multivalent vaccine against cecal colonization of Campylobacter jejuni in commercial chickens. Google Scholar link: https://scholar.google.com/citations?user=c6f8QtwAAAAJ&hl=en
Discipline
Bacteriology Cellular biology Immunology – B-cells Immunology – T-cells Molecular biology Protein biology
Host species
Poultry
Pathogen
Bacteria›E. coli Bacteria›Salmonella
Stage of vaccine development
Antigen discovery and immunogen design Vaccine delivery