Currently, my work focuses on developing an optimized high-throughput protocol for the generation and isolation of bioengineered bacterial extracellular vesicles (BEVs) from GRAS-graded LAB vectors. Structural and functional characterization of naturally secreted BEVs and bioengineered BEVs. Along with this, I have tried to optimize the BEV biogenesis by altering bacterial growth culture microenvironments. For the application of bioengineered BEVs as Antigen Presentation Platforms, we chose model pathogen C. jejuni which commensally colonizes in chicken gut, and is associated with Gastroenteritis, septicemia, and Guillain barre syndrome in human. It suggests a potential application in vaccine development or immunotherapy. By utilizing BEVs to deliver antigens, might enhance immune responses and potentially develop more effective strategies for combating pathogens.