Dr
Chamith
Hewawaduge
Dr
Chamith
Hewawaduge
Research Scientist
Sri Lanka Institute of Biotechnology
Biography
I have specialized in researching and developing vaccines for veterinary and zoonotic diseases, focusing on utilizing live bacterial vaccine platforms and identifying virulence genes in Salmonella species. Furthermore, I possess expertise in creating live attenuated Salmonella strains through lambda red recombination, which can be employed to deliver vaccine antigens for diseases such as SARS-CoV2, Avian Influenza, and Porcine epidemic diarrhea virus. Subsequently, I conduct thorough testing to assess the effectiveness of these vaccines in various animal models, ensuring they provide adequate protection against these diseases. As a highly motivated and creative individual, I highly value teamwork and possess strong leadership abilities. My unwavering commitment and pursuit of excellence drive me to carry out all tasks dedicatedly. I am keen on advancing my research career by applying my skills and knowledge to gain valuable experience. Research interests
During my undergraduate studies in microbiology, I developed a deep interest in vaccinology as I grappled with the complexities of creating vaccines that are both safe and potent in combating infectious diseases. With a Master's and a PhD in preventive veterinary medicine, I have established a robust expertise in formulating vaccines for veterinary diseases. My passion for this field was further cultivated during my PhD as a researcher in Professor Lee John Hwa's laboratory at the College of Veterinary Medicine, Jeonbuk National University in South Korea, where I specialized in developing vaccine platforms.
During my postgraduate studies, I have authored 8 research articles as the first author and contributed to 31 co-authored research articles. I have also presented numerous posters and oral presentations at international Microbiology, Immunology, and Biomedical conferences from 2017 to 2023. My work has garnered 360 citations on Google Scholar and maintains a H-index of 9. My expertise spans over seven years and encompasses molecular cloning, expression profiling in both prokaryotic and eukaryotic cells, cell culture (involving primary cell isolation and culture, as well as eukaryotic cell lines), development of cancer models, immunoblotting, E. coli expression system for recombinant protein purification, immunofluorescence assay, ELISA, and Lambda red recombination technique. Additionally, I have extensive experience in conducting in-vivo and in-vitro experiments with BSL2 and BSL3 laboratory-level viruses and bacteria, such as SARS-CoV2, SFTSV, Influenza, PEDV, IBV, Brucella, and others.
Currently, I am working as a research scientist at the Sri Lanka Institute of Biotechnology (SLIBTEC) and embarking on developing an inactivated rabies vaccine, which is anticipated to benefit the veterinary sector and contribute significantly to the country's overall economic growth. My primary focus is to detect zoonotic diseases of major concern and analyze the existing approaches and obstacles in creating vaccines for these animal diseases. Additionally, I intend to delve into the concepts of customizing vaccine platforms for particular animal diseases and assess the potential influence of these specialized vaccine platforms on public health.
Projects you're working on
Development of vaccine strategy against rabies.
Development of an adenoviral vector-based bait vaccine against rabies Discipline
Immunology – B-cells Immunology – T-cells Immunology – innate Virology Host species
Dogs Poultry Small ruminants Zoonoses Pathogen
Bacteria Bacteria›E. coli Bacteria›Salmonella Viruses Viruses›Coronavirus Viruses›Foot-and-mouth disease virus Viruses›Influenza virus Viruses›Rabies virus Stage of vaccine development
Antigen discovery and immunogen design Field trials Pre-clinical trials Vaccine delivery