Dr Saengchan Senapin

National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand

Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, Bangkok 10400, Thailand

Telephone: (662) 2015867, Fax: (662) 3547344, E-mail: saengchan@biotec.or.th                         

 

Expertise

Surveillance and molecular characterization of emerging and re-emerging pathogens in fish and shrimp
Protein interaction study between shrimp and viral proteins

Academic Awards

L’Oreal Thailand Fellowship “For Women in Science 2011 (Life Science)”
Taguchi Outstanding Researcher award 2016

 

Publications (2014-2018)

Dong, H.T., Senapin, S., Jeamkunakorn, C., Nguyen, V.V., Nguyen, N.T., Rodkhum, C., Khunrae, P., Rattanarojpong, T. (2019) Natural occurrence of edwardsiellosis caused by Edwardsiella ictaluri in farmed hybrid red tilapia (Oreochromis sp.) in Southeast Asia. Aquaculture. 499: 17-23. Senapin, S., Dong, H.T., Meemetta, W., Gangnonngiw, W., Sangsuriya, P., Vanichviriyakit, R., Sonthi, M., Nuangsaeng, B. (2019) Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia. J. Fish. Dis. In press Wangman, P., Longyant, S., Taengchaiyaphum, S., Senapin, S., Sithigorngul, P. and Chaivisuthangkura, P. (2018) PirA & B toxins discovered in archived shrimp pathogenic Vibrio campbellii isolated long before EMS/AHPND outbreaks. Aquaculture 497: 494-502 Sangsuriya, P., Charoensapsri, W., Sutthangkul, J., Senapin, S., Hirono, I., Tassanakajon, A. and Amparyup, P. (2018) A novel white spot syndrome virus protein WSSV164 controls prophenoloxidases, PmproPOs in shrimp melanization cascade. Dev Comp Immunol. 86: 109–117 Saengphan, N., Bhinyo, P., Senapin, S., Laosinchai, P., Ruenwongsa, P., Suksomnit, A., Phiwsaiya, K. (2018) Morphology and molecular phylogeny of Macrobrachium suphanense sp. nov. (Decapoda: Palaemonidae) from Thailand. Zootaxa. 4482:151-163. Pattanapon, K., Dong, T.H., Hirono, I., Kondo, H., Senapin, S., Rodkhum, C. (2018) Genome characterization of piscine 'Scale drop and Muscle Necrosis syndrome'-associated strain of Vibrio harveyi focusing on bacterial virulence determinants. J Appl Microbiol. doi: 10.1111/jam.13676. Dong, H.T., Senapin, S., Phiwsaiya, K., Techatanakitarnan, C., Dokladda, K., Ruenwongsa, P., Panijpan, B. (2018) Histopathology and culturable bacteria associated with “big belly” and “skin nodule” syndromes in ornamental Siamese fighting fish, Betta splendens, Microb Pathog. 122: 46-52. Mata, W., Putita, C., Dong, H.T., Kayansamruaj, P., Senapin, S., Rodkhum, C. (2018) Quinolone-resistant phenotype of Flavobacterium columnare isolates harbored point mutations in both parC and gyrA but not in either gyrB or parE. J Glob Antimicrob Resist. 15:55-60. Sotanon, N., Saleeart, A., Rattanarojpong, T., Dong, H.T., Senapin, S., Wongprasert, K., Sarikavanij, S., Khunrae, P. (2018) C-terminal domain of WSSV VP37 is responsible for shrimp haemocytes binding which can be inhibited by sulfated galactan. Fish Shellfish Imm.  77: 312-318. Senapin, S., Shyama, K.U., Meemetta, W., Rattanarojpong, T., Dong, H.T. (2018) Inapparent infection cases of tilapia lake virus (TiLV) in farmed tilapia. Aquaculture. 487: 51-55. Srisuk, C., Senapin, S., Bendena, W.G., Longyant, S., Sithigorngul, P. Chaivisuthangkura, P. (2018) Molecular isolation and characterization of a haemocyanin of Macrobrachium rosenbergii reveal its antibacterial activities. Aquaculture Res. 49: 505–516. Ataguba, G.A., Dong, H.T., Rattanarojpong, T., Senapin, S., Salin, K.R. (2018) Piper betle leaf extract inhibits multiple aquatic bacterial pathogens and in vivo Streptococcus agalactiae infection in Nile tilapia. Turk J Fish Aquat Sc. 18: 671-680 Phiwsaiya, K., Charoensapsri, W., Taengphu, S., Dong, H.T., Sangsuriya, P., Nguyen, G.T.T., Pham, H.Q., Amparyup, P., Sritunyalucksana, K., Taengchaiyaphum, S., Chaivisuthangkura, P., Longyant, S., Sithigorngul, P., Senapin, S. (2017) A natural Vibrio parahaemolyticus ΔpirAVp pirBVp+ mutant kills shrimp but produces no PirVp toxins or acute hepatopancreatic necrosis disease Lesions. Appl. Environ. Microbiol. 83: e00680-17 Jariyapong, P., Pudgerd, A., Weerachatyanukul, W., Hirono, I., Senapin, S., Dhar, A.K., Chotwiwatthanakun, C. (2017) Construction of an infectious Macrobrachium rosenbergii nodavirus from cDNA clones in Sf9 cells and improved recovery of viral RNA with AZT treatment. Aquaculture. 483: 111-119. Dong, H.T., Jitrakorn, S., Kayansamruaj, P., Pirarat, N., Rodkhum, C., Rattanarojpong, T., Senapin, S., Saksmerprome, V. (2017) Infectious spleen and kidney necrosis disease (ISKND) outbreaks in farmed barramundi (Lates calcarifer) in Vietnam. Fish Shellfish Imm. 68: 65-73. Dong, H.T., Ataguba, G.A., Khunrae, P., Rattanarojpong, T., Senapin, S. (2017) Evidence of TiLV infection in tilapia hatcheries from 2012 to 2017 reveals probable global spread of the disease. Aquaculture 479:579-583. Kayansamruaj, P., Dong, H.T., Hirono, I., Kondo, H., Senapin, S., Rodkhum, C. (2017) Comparative genome analysis of fish pathogen reveals extensive sequence diversity within the species, Infect. Genet. Evol. 54: 7-17 Wangman, P., Chaivisuthangkura, P., Sritunyalucksana, K., Taengchaiyaphum, S., Senapin, S., Pengsuk, C., Sithigorngul, P., Longyant, S. (2017) Development of monoclonal antibodies specific to ToxA and ToxB of Vibrio parahaemolyticus that cause acute hepatopancreatic necrosis disease (AHPND). Aquaculture. 474: 75–81 Dong, H.T., Siriroob, S., Meemetta, W., Santimanawong, W., Gangnonngiw, W., Pirarat, N., Khunrae, P., Rattanarojpong, T., Vanichviriyakit, R., Senapin, S. (2017). Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection. Aquaculture 476: 111-118 Dong, H.T., Techatanakitarnan, C., Jindakittikul, P., Thaiprayoon, A., Taengphu, S., Charoensapsri, W., Khunrae, P., Rattanarojpong, T., Senapin, S. (2017) Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.). J. Fish. Dis. 40: 1395-1403 Dong, H.T., Taengphu, S., Sangsuriya, S., Charoensapsri, W., Phiwsaiya, P., Sornwatana, T., Khunrae, P., Rattanarojpong, T., Senapin, S. (2017) Recovery of Vibrio harveyi from scale drop and muscle necrosis disease in farmed barramundi, Lates calcarifer in Vietnam. Aquaculture. 473: 89–96 Dong, H.T., Nguyen, V.V., Mata, W., Kayansamruaj, P., Senapin, S., Nilubol, D., Rodkhum, C. (2016) Diversity of non-Flavobacterium columnare bacteria associated with columnaris-like diseased fish. Thai J Vet Med. 46:251-259 Dong, H.T., Gangnonngiw, W., Phiwsaiya, K., Charoensapsri, W., Nguyen, V.V., Nilsen, P., Pradeep, P.J., Withyachumnarnkul, B., Senapin, S., Rodkhum, C. (2016) Duplex PCR assay and in situ hybridization for detection of Francisella spp. and Francisella noatunensis subsp. orientalis in red tilapia. Dis Aquat Org. 120:39-47  Dong, H.T., Nguyen, V.V., Kayansamruaj, P., Gangnonngiw, W., Senapin, S., Pirarat, N., Nilubol, D., Rodkhum, C. (2016) Francisella noatunensis subsp. orientalis infects striped catfish (Pangasianodon hypophthalmus) and common carp (Cyprinus carpio) but does not kill the hosts. Aquaculture. 464: 190–195  Peepim, T., Dong, H.T., Senapin, S., Khunrae, P., Rattanarojpong, T. (2016) Epr3 is a conserved immunogenic protein among Aeromonas species and able to induce antibody response in Nile Tilapia. Aquaculture 464: 399–409  Phairoh, P., Suthibatpong, T., Rattanarojpong, T., Jongruja, N., Senapin, S., Choowongkomon, K., Khunrae, P. (2016) ICP35 Is a TREX-Like protein identified in white spot syndrome virus. PLoS ONE 11(6): e0158301. doi:10.1371/journal.pone.0158301  Saleeart, A., Mongkorntanyatip, K., Sangsuriya, P., Senapin, S., Rattanarojpong, T., Khunrae, P. (2016) The interaction between PmHtrA2 and PmIAP and its effect on the activity of Pm caspase. Fish Shellfish Imm. 55:393–400  Senapin, S., Dong, H.T., Meemetta, W., Siriphongphaew, A., Charoensapsri, W., Santimanawong, W., Turner, W.A., Rodkhum, C., Withyachumnarnkul, B., Vanichviriyakit, R. (2016) Hahella chejuensis is the etiological agent of a novel red egg disease in tilapia (Oreochromis spp.) hatcheries in Thailand. Aquaculture. 454:1-7 Suthangkornkul, R., Sriworanun, P., Nakai, H., Okuyama, M., Svasti, J., Kimura, A., Senapin, S., Arthan, D. (2016) A Solanum torvum GH3 β-glucosidase expressed in Pichia pastoris catalyzes the hydrolysis of furostanol glycoside. Phytochemistry. 127:4-11. Pradeep, P.J., Sirthammajak, S., Kampeera, J., Suebsing, R., Turner, W.A., Senapin, S., Withyachumanarnkul, B. (2016) Evidence of vertical transmission and tissue tropism of Streptococcosis from naturally infected red tilapia (Oreochromis spp.) Aquaculture Reports 3:58-66 Peepim, T., Phiwsaiya, K., Charoensapsri, W., Khunrae, P., Senapin, S., Rattanarojpong, T. (2016) Knockdown of Litopenaeus vannamei HtrA2, an upregulated gene in response to WSSV infection, leading to delayed shrimp mortality. J Biotechnol. 219:48-56 Wangman, P., Longyant, S., Utari, H. B., Senapin, S., Pengsuk, C., Sithigorngul, P., Chaivisuthangkura, P. (2016). Sensitivity improvement of immunochromatographic strip test for infectious myonecrosis virus detection. Aquaculture. 453: 163–168 Nguyen, V.V, Dong, H.T Senapin, S., Pirarat, N., Rodkhum, C. (2016) Francisella noatunensis subsp. orientalis, an emerging bacterial pathogen affecting cultured red tilapia (Oreochromis sp.) in Thailand. Aquac Res. 47: 3697-3702 Dong, H.T., Senapin, S., LaFrentz, B., Rodkhum, C. (2016) Virulence assay of rhizoid and non-rhizoid morphotypes of Flavobacterium columnare in red tilapia, Oreochromis sp., fry. J Fish Dis. 39:649-655 Thammasorn, T., Sangsuriya, P., Meemetta, P., Senapin, S., Jitrakorn, S., Rattanarojpong, T., Saksmerprome, V. (2015) Large-scale production and antiviral efficacy of multi-target double-stranded RNA for the prevention of white spot syndrome virus (WSSV) in shrimp. BMC Biotechnol. 15:110 Suebsing, R., Kampeera, J., Sirithammajak, S., Pradeep, P.J., Jitrakorn, S., Arunrut, N., Sangsuriya, P., Saksmerprome, V., Senapin, S., Withyachumnarnkul, B., Kiatpathomchai, W. (2015) Shewanella putrefaciens in cultured tilapia detected by a new calcein-loop-mediated isothermal amplification (Ca-LAMP) method. Dis Aquat Organ. 117: 133–143 Sangsuriya, P., Charoensapsri, W., Chomwong, S., Senapin, S., Tassanakajon, A., Amparyup P. (2016) A shrimp pacifastin light chain-like inhibitor: Molecular identification and role in the control of the prophenoloxidase system. Dev Comp Immunol. 54: 32-45 Apitanyasai, K., Amparyup, P., Charoensapsri, W., Senapin, S., Tassanakajon, A. (2015) Role of Penaeus monodon hemocyte homeostasis associated protein (PmHHAP) in regulation of caspase-mediated apoptosis. Dev Comp Immunol. 53: 234–243 Dong, H.T., Nguyen, V.V., Le, H.D., Sangsuriya, P., Jitrakorn, S., Saksmerprome, V., Senapin, S., Rodkhum, C. (2015) Naturally concurrent infections of bacterial and viral pathogens in disease outbreaks in cultured Nile tilapia (Oreochromis niloticus) farms. Aquaculture. 448: 427–435 Youngcharoen, S. Senapin, S., Lertwimol, T., Longyant, S., Sithigorngul, P., Flegel, T.W., Chaivisuthangkura, P. (2015) Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of Macrobrachium rosenbergii nodavirus reveals their roles in apoptosis. Fish Shellfish Imm. 45: 534-542 Dong, H.T., Nguyen, V.V., Phiwsaiya, K, Gangnonngiw, W., Withyachumnarnkul, B., Rodkhum, C., Senapin, S. (2015) Concurrent infections of Flavobacterium columnare and Edwardsiella ictaluri in striped catfish, Pangasianodon hypophthalmus in Thailand. Aquaculture. 448: 142–150 Panijpan, B., Laosinchai, P., Senapin, S., Kowasupat, C., Ruenwongsa, P., Kühne, J., Phiwsaiya, K. (2015) Mitochondrial COI and nuclear RAG1 DNA sequences and analyses of specimens of the three morphologically established species in the genus Trichopsis (Perciformes: Osphronemidae) reveal new/cryptic species. Meta Gene. 4: 17-28 Charoensapsri. W., Sangsuriya, P., Lertwimol, T., Gangnonngiw, W., Phiwsaiya, K., Senapin, S. (2015) Laminin receptor protein is implicated in hemocyte homeostasis for the whiteleg shrimp Penaeus (Litopenaeus) vannamei. Dev Comp Immunol. 51: 39-47 Jearaphunt, M., Amparyup, P., Sangsuriya, P., Charoensapsri, W., Senapin, S., Tassanakajon, A. (2015) Shrimp serine proteinase homologues PmMasSPH-1 and -2 play a role in the activation of the prophenoloxidase system. PLoS ONE. DOI: 10.1371/journal.pone.0121073 Sutthangkul, J., Amparyup, P., Charoensapsri, W., Senapin, S., Phiwsaiya, K., Tassanakajon A. (2015) Suppression of shrimp melanization during white spot syndrome virus infection. J Biol Chem. 290: 6470-6481 Namvongsakool, P., Asuvapongpatana, S., Senapin, S., Weerachatyanukul, W., Treerattrakool, S., Withyachumnarnkul, B. (2015) A novel localization of molt-inhibiting hormone in the tegumental glands of shrimp Penaeus monodon and its possible role in shrimp molting. Aquaculture. 438: 129-137 Panijpan, B., Kowasupat, C., Laosinchai, P., Ruenwongsa, P., Phongdara, A., Senapin, S., Wanna, W., Phiwsaiya, K, Kühne, J., Fasquel, F. (2014) Southeast Asian mouth-brooding Betta fighting fish (Teleostei: Perciformes) species and their phylogenetic relationships based on mitochondrial COI and nuclear ITS1 DNA sequences and analyses. Meta Gene. 2: 862-879 Suraprasit, S., Methatham, T., Jaree, P., Phiwsaiya, K., Senapin, S., Hirono, I., Lo, C.F., Tassanakajon, A., Somboonwiwat, K. (2014) Anti-lipopolysaccharide factor isoform 3 from Penaeus monodon (ALFPm3) exhibits antiviral activity by interacting with WSSV structural proteins. Antiviral Res. 110:142-150 Udompetcharaporn, A., Junkunlo, K., Senapin, S., Roytrakul, S., Flegel, T.W., Sritunyalucksana, K. (2014) Identification and characterization of a QM protein as a possible peptidoglycan recognition protein (PGRP) from the giant tiger shrimp Penaeus monodon. Dev Comp Immunol. 46:146-154 Sangsuriya, P., Huang, JY., Chu, YF., Phiwsaiya, K., Leekitcharoenphon, P., Meemetta, W., Senapin, S., Huang, WP., Withyachumnarnkul, B., Flegel, TW., Lo, CF. (2014) Construction and application of a protein interaction map for white spot syndrome virus (WSSV). Mol Cell Proteomics. 13(1):269-282. doi: 10.1074/mcp.M113.029199 Lertwimol, T., Sangsuriya, P., Phiwsaiya, K., Senapin, S., Phongdara, A., Boonchird, C., Flegel, T.W. (2014) Two new anti-apoptotic proteins of white spot syndrome virus that bind to an effector caspase (PmCasp) of the giant tiger shrimp Penaeus (Penaeus) monodon. Fish Shellfish Imm. 38:1-6 Senapin, S., Phiwsaiya, K., Laosinchai, P., Kowasupat, C., Ruenwongsa, P., Panijpan, B. (2014) Phylogenetic analysis of parasitic trematodes of the genus Euclinostomum found in Trichopsis and Betta Fish. J Parasitology. 100(3): 368-371 Sangsuriya, P., Phiwsaiya, K., Pratoomthai, B., Sriphaijit, T., Amparyup, P., Withyachumnarnkul, B., Senapin, S. (2014) Knockdown of a novel G-protein pathway suppressor 2 (GPS2) leads to shrimp mortality by exuvial entrapment during ecdysis. Fish Shellfish Imm. 37:46-52 Srisuk, C., Longyant, S., Senapin, S., Sithigorngul, P., Chaivisuthangkura, P. (2014) Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii. Fish Shellfish Imm. 36:552-562 Kowasupat, C., Panijpan, B., Laosinchai, P., Ruenwongsa, P., Phongdara, A., Wanna, W., Senapin, S., Phiwsaiya, K. (2014) Biodiversity of the Betta smaragdina (Teleostei: Perciformes) in the northeast region of Thailand as determined by mitochondrial COI and nuclear ITS1 gene sequences. Meta Gene. 2: 83-95